Figure 2. Analysis of purified heterodimeric sGC with an amino-terminally tagged β₁ subunit.

Coomassie blue staining (A) and Western blot analysis with antibodies directed against the α₁ subunit (B) and the β₁ subunit (C) using cytosolic fraction (lane 1) or purified sGC (lane 2). Absorption spectra of purified sGC under basal conditions (solid line) and in the presence of 100 µM DEA/NO (dotted line) (D). Specific sGC activity of purified sGC assayed with Mg²⁺ (black column) and Mn²⁺ (grey column) as cofactor and in the presence of 100 µM DEA/NO (white column) and 10 µM Cinaciguat (dark grey column) (E).