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. 2010 Jul 15;5(7):e11607. doi: 10.1371/journal.pone.0011607

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Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Effects of DCB-3503 and CHX on translation of uncapped and capped poly(A) tail cyclin D1 mRNAs in HeLa cell free lysate system. B. Effects of DCB-3503, CHX, and rapamycin on translation of capped luciferase mRNAs with or without 3'poly(A) tail in HeLa cell free lysate system. Values are depicted as mean ± S.D. from at least three separate experiments. C. Western blot analysis of phosphorylated p70 S6Kinase T³⁸⁹, S6 S^235/236, phosphorylated 4EBP1 S⁶⁵ and T^37/46 under DCB-3503 and rapamycin treatment for 2 hours in HepG2 cells. 4EBP1 and β-Actin blots were used as internal loading controls. A and C are representatives from three separate experiments.