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A synchronized fusion assay was performed as described for Fig. 4B, the cells were fixed at t-60, stained for (A) LAMP-1, (B) mannose-6-phosphate receptor, (C) Tfn-TR, or (D) Rab11 and then examined by LSCM (Bars = 10 µm). Percent colocalization of MLG with the indicated marker from approximately 20 individual cells from two independent experiments is shown in the upper right-hand corner of the merged images.
