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. Author manuscript; available in PMC: 2010 Jul 15.
Published in final edited form as: Nature. 2009 Dec 17;462(7275):935–939. doi: 10.1038/nature08657

Figure 4. Linkage between PIAS1/4 and RNF8/168.

Figure 4

a, U2OS cells were treated and probed as indicated. b,c, As (a) except cells stably expressed GFP-RNF8 or GFP-BRCA1/Flag-BARD1; see Supplementary Figs. 9a and 9b for quantifications. d, U2OS cells were treated and probed as indicated; see Supplementary Figs. 9d 10c for quantifications and siRNA efficiencies, respectively. e,f, U2OS cells stably expressing GFP-PIAS1 (e) or RFP-PIAS4 (f) were treated and probed as indicated. g, Model; dashed arrows indicate protein requirements for accumulation, solid arrows indicate target-protein modifications.