Table 1.
Gene Tr | Accession No. | Forward primer | Reverse primer | Length (bp) |
---|---|---|---|---|
D1A | AB372107 | 5′-CCATCATGATAGTAACCTACA-3′ | 5′-GAATTAGCCCATCCAAACCA-3′ | 320 |
D1B | AB372108 | 5′-GTRGCCTTYGAYATYATGTGCTC-3′ | 5′-ARAANGGBARCCARCARCACAC-3′ | 625 |
D1D | AB372109 | 5′-GTRGCCTTYGAYATYATGTGCTC-3′ | 5′-ARAANGGBARCCARCARCACAC-3′ | 478 |
D2 | AB490795 (for v1) | 5′-TGYGCCATCAGCRTNGACAGGT-3′ | 5′-GCRCTRTTSACRTARCCHAGCCA-3′ | 851 |
D2 | AB372110 (for v5) | 5′-TGYGCCATCAGCRTNGACAGGT-3′ | 5′-GCRCTRTTSACRTARCCHAGCCA-3′ | 701 |
D3 | AB372111 | 5′-TGYGCCATCAGCRTNGACAGGT-3′ | 5′-GCRCTRTTSACRTARCCHAGCCA-3′ | 780 |
D4 | GQ359780 | 5′-ATCCTCCTCATCCTCCTTATCGT-3′ | 5′-ACGGTGTAAATGATGGGGTTGA-3′ | 687 |
Listed are the cloned dopamine receptor cDNA gene transcripts (Gene Tr), their accession Nos., the forward and reverse primers used to clone them, and the clone lengths. The primers for D1B and D1D as well for D2 v1, D2 v5, and D3 receptors were identical.