Figure 6.

CaMKII subunit levels in CA1 PSD-enriched subcellular fractions as a function of time after drug withdrawal. Representative immunoblots of total CaMKIIα, pThr²⁸⁶-CaMKIIα, and CaMKIIβ protein with their respective GAPDH loading controls are shown in PSD-enriched subfractions of CA1 minislices from (a) 1-day and (b) 2-day FZP-withdrawn rats in the leftmost panels. Histograms of integrated signal density as a percent of the paired control density (n=4–7 lanes/group) are shown to the right. Total-CaMKIIα expression levels were significantly increased in 2-day FZP-withdrawn rats. No significant changes in pThr²⁸⁶-CaMKIIα or CaMKIIβ expression levels were found. Asterisks denote p<0.05.