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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: Free Radic Biol Med. 2010 Jun 16;49(4):667–673. doi: 10.1016/j.freeradbiomed.2010.05.028

Figure 1. Tempol reduces the number of mature 3T3-L1 adipocytes and inhibits proliferation.

Figure 1

3T3-L1 cells were differentiated in the presence and absence of 1 or 2 mM Tempol. Six days after initiation of differentiation cells were fixed, stained with FITC-conjugated perilipin (green), counterstained with PI (red) and counted: photos of undifferentiated (1A), differentiated (1B), 1 mM Tempol-treated differentiated 3T3-L1 cells (1C) and 2 mM Tempol-treated-differentiated 3T3-L1 cells (1D). (1E) The ratio of FITC-conjugated perilipin positive stained cells to total number of cells decreased upon treatment with Tempol. (1F) Duplicate plates were trypsinized and counted and relative cell growth is shown. 1 mM Tempol partially inhibited 3T3-L1 proliferation, while 2 mM Tempol completely inhibited proliferation. Data presented as mean ± SEM of 4–7 independent experiments (1F).