Fig. 3.

Fluoro-Jade B (FJB) staining for degenerating neurons in the contralesional (Contra), ipsilesional (Ipsi) and sham-operated (Sham) cortices. The open rectangle in each lower magnification image (A–D, I) indicates the region shown in the accompanying higher magnification image (E–H, J). FJB (+) neurons were abundant in the peri-infarct area on post-lesion day 1 (B and F). They were less abundant by post-lesion day 3 (D and H). Clearly labeled neurons were rarely found in either the contralesional cortex (E and G) or in sham-operates (J). In quantitative analyses (K), there were significantly more FJB (+) neurons in the peri-infarct region at each time point compared with the contralateral side (*p<0.001 vs. Contra). There were significantly fewer FJB+ cells in peri-infarct cortex at Day 3 compared with Day 1 (†p <0.001 vs. Day 1 Ipsi). There was no significant difference in the contralesional cortex between two time points.