Table 2.
Fold changes in expression (FC) (log2) of genes encoding enzymes involved in the primary metabolism of nematode-induced syncytia were studied by quantitative RT-PCR at 5, 10 and 15 dai and compared with GeneChip data (5 + 15 dai)
5 dai | 10 dai | 15 dai | GeneChip data* | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Locus | EC | FC | SE | FC | SE | FC | SE | FC | Gene name | Description | Metabolism |
At1g12900 | 1.2.1.9 | 1.83 | 0.46 | 6.30 | 2.26 | 11.52 | 6.24 | 4.6 | GAPA-2 | Glyceraldehyde 3-phosphate dehydrogenase A, subunit 2 | Glyc |
At3g23940 | 4.2.1.9 | 4.22 | 0.35 | 7.94 | 2.19 | 7.59 | 2.14 | 4.0 | AADH | Dihydroxy acid dehydratase | AA |
At2g30970 | 2.6.1.1 | 2.42 | 0.18 | 3.53 | 0.17 | 2.91 | 0.45 | 4.3 | ASP1 | Aspartate amino transferase 1 | AA |
At4g32520 | 2.1.2.1 | 1.68 | 0.40 | 2.29 | 0.72 | 2.81 | 0.71 | 3.5 | SHM3 | Serine hydroxymethyl transferase 3 | AA |
At1g35910 | 3.1.3.12 | 3.28 | 0.84 | 2.89 | 1.61 | 2.68 | 1.72 | 2.8 | TPPd | Trehalose phosphate phosphatase | S |
At3g54090 | 2.7.1.4 | 2.08 | 0.13 | 3.65 | 0.29 | 2.59 | 0.10 | 2.4 | FK | Fructokinase | S |
At3g63250 | 2.1.1.14 | 2.08 | 0.72 | 2.06 | 0.75 | 2.57 | 0.33 | 2.3 | HMT2 | Homocysteine methyltransferase 2 | AA |
At1g69370 | 5.4.99.5 | 1.94 | 0.57 | 1.85 | 0.20 | 2.20 | 0.63 | 2.7 | CM3 | Chorismate mutase | AA |
At5g14590 | 1.1.1.42 | 1.87 | 0.17 | 2.44 | 0.41 | 2.09 | 0.30 | 2.2 | IDH | Isocitrate dehydrogenase | TCA |
At4g29840 | 4.2.3.1 | 1.31 | 0.48 | 1.51 | 0.33 | 1.67 | 0.19 | 3.1 | TS/MTO2 | Threonine synthase | AA |
At4g02610 | 4.2.1.20 | 1.34 | 0.24 | 1.36 | 0.18 | 1.58 | 0.23 | 3.3 | TSA1 | Tryptophan synthase, α subunit | AA |
The Gene Chip data have been published previously (Szakasits et al., 2009). Values are means ± SE (n = 3).
AA, amino acid metabolism; Glyc, glycolysis; S, sugar metabolism; TCA, tricarboxylic acid cycle.