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. 2010 May 27;11(6):2322–2335. doi: 10.3390/ijms11062322

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© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 2. — The Semi-Quantitative Polymerase Chain Reaction (SQ-PCR) analysis of Muc1 mRNA in porcine tissues (Day 24 of pregnancy). (A) mRNA products of SQ-PCR analysis. A single amplified fragment of 553 bp was detected for Muc1 and 452 bp for GAPDH. GAPDH was amplified to quantify and test quality of cDNA. M: 100 bp molecular Marker. Expected fragment length (bp) is indicated on the right. (B) Relative abundance of Muc1 mRNA normalized to GAPDH. 1: heart; 2: spleen; 3: kidney; 4: spinal cord; 5: bladder; 6: adrenal gland; 7: brain; 8: cerebellum; 9: pituitary; 10: hypothalamus; 11: back subcutaneous fat; 12: skeletal muscle; 13: ovary; 14: oviduct; 15: body of uterus; 16: cervix; 17: endometrium (at attachment sites); 18: endometrium (between attachment sites); 19: embryo; 20: liver; 21: lung; 22: large intestine.