Table 2.
Oligonucleotide primers used for semi-quantitative PCR (SQ-PCR) and real-time PCR (RT-PCR) of porcine Muc1 and a house keeping gene.
| Primer Name | Primer Sequences (5′–3′) | Annealing Temperature (°C) | Product Size (bp) | Genbank Accession no./references |
|---|---|---|---|---|
| SQ-PCR | ||||
| Muc1-1 | Forward:CACCACCAGCTACTACAAGG | 62 | 553 | AY243508 |
| Reverse:TGCCAGGTTCGAGTAAGAG | ||||
| GAPDH-1 | Forward:ACCACAGTCCATGCCATCAC | 60 | 452 | AF017079/BDBiosciences |
| Reverse:TCCACCACCCTGTTGCTGTA | ||||
| RT-PCR | ||||
| Muc1-2 | Forward:GTGCCGACGAAAGAACTG | 60 | 187 | AY243508 |
| Reverse:TGCCAGGTTCGAGTAAGAG | ||||
| GAPDH-2 | Forward:GTCCACTGGTGTCTTCACGA | 60 | 154 | AF141959/[29] |
| Reverse:GCTGACGATCTTGAGGGAGT |