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. 2010 Jul 15;24(14):1546–1558. doi: 10.1101/gad.573310

Figure 2.

Figure 2.

Pre-rRNA synthesis defects increase in top1Δ mutant at low growth temperatures. Total RNA from wild-type and single top1Δ strains grown at 18°C, 25°C, or 30°C was resolved on an agarose gel and analyzed by Northern hybridization, using probes 130 (A), 003 (B,C,E), and 004 (D). (F) Cytoplasmic SCR1 RNA was used as a loading control. C shows a stronger exposure of the lower region (below 18S) of B. Intact pre-rRNAs and truncated pre-rRNA fragments are labeled. “(18S)” and “(25S)” indicate the position of migration of 18S and 25S rRNAs. Probe names are bracketed (see Fig. 1A for location of probes).