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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: J Immunol. 2009 Jul 22;183(4):2435–2443. doi: 10.4049/jimmunol.0900568

FIGURE 3.

FIGURE 3

IL-27 inhibits IL-17 production from total CD4+ T cells. A, Total CD4+ T cells were stimulated with anti-CD3 and anti-CD28 in the presence or absence of IL-27 for 3 days and cell-free culture supernatants were assayed for IL-17A by ELISA. B, IL-27 inhibits RORC in total CD4+ T cells. Quantitative PCR of the expression of mRNA total CD4+ T cells stimulated with plate-bound anti-CD3 and anti-CD28 in the presence or absence of IL-27 (100 ng/ml). C–G, IL-27 inhibits molecules associated with Th17 effector molecules in total CD4+ T cells. Quantitative PCR of the expression of mRNA encoding IL-17F, IL-22, IL-23R, CCR6, and CCL20 from total CD4+ T cells stimulated with plate-bound anti-CD3 and anti-CD28 in the presence or absence of IL-27 (100 ng/ml). Data are from five randomly selected healthy donors. Horizontal bars indicate the median. H and I, IL-27-mediated IL-17 suppression was mediated by IFN-γ. Total CD4+ T cells stimulated with plate-bound anti-CD3 and anti-CD28 in the presence or absence of IL-27 (100 ng/ml). To some conditions, neutralizing anti-IL-10 Ab (20 μg/ml) or anti-IFN-γ (20 μg/ml) was added as indicated. Data represent one of three independent experiments with cells from two randomly selected donors.