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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: J Immunol. 2009 Jul 22;183(4):2435–2443. doi: 10.4049/jimmunol.0900568

FIGURE 5.

FIGURE 5

IL-27 inhibits IL-17 polarizing cytokines from monocyte derived dendritic cells. A–C, Cytometric bead assay of IL-1β and IL-6 and ELISA of IL-23 in monocyte-derived DCs stimulated with LPS or PGN alone or in the presence of IL-27 (100 ng/ml). D, DCs express IL-27R on their surface. Monocyte-derived DCs used in our experiments were >95% CD11c+ and these cells expressed IL-27R on their surface as determined by flow cytometry. E, Total CD4+ T cells were cultured with a 1: 3 ratio of DCs prestimulated with LPS or PGN alone or in the presence of IL-27. The DCs were washed three times before they were cultured with T cells. After 4 days of coculture, the cell-free culture supernatants were assayed for IL-17 by ELISA. Data are representative of three experiments with three donors.