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. 2010 May 12;30(19):6646–6657. doi: 10.1523/JNEUROSCI.5428-09.2010

Figure 7.

Figure 7.

EHD1 oligomerization is necessary for inhibition of NgCAM endocytosis. A, Schematic diagram of the domain organization of EHD1. The position of the V203 mutant is indicated. B, Endocytosis of NgCAM (after 20 min of anti-NgCAM antibody uptake; red) in the somatodendritic domain in a neuron coexpressing myc–EHD1V203P (green). Endocytosis of NgCAM is high in cells expressing myc–EHD1V203P. C, The percentage of neurons showing endocytic uptake of NgCAM was determined for 150–200 cells each in four independent experiments. The oligomerization-impaired EHD1V203P mutant does not impair NgCAM endocytosis. Error bars indicate SEM *p < 0.01, statistically significant from GFP. D, A/D PI of surface NgCAM was determined for neurons coexpressing myc–EHD1, or myc–V203P EHD1 or GFP as control. Error bars indicate SEM. **p < 0.0001, statistically significant from GFP. n = 32 for myc–EHD1 and 25 for myc–V203P EHD1. The expression range of the two myc-tagged constructs is comparable (data not shown).