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. 2010 Jul 21;99(2):489–498. doi: 10.1016/j.bpj.2010.04.027

Figure 1.

Figure 1

Overview on HA constructs and synthetic peptides. (A and B) Rhodamine (TAMRA) labeled full length HA (A, Rh-HA) or synthetic peptide (TMD-peptide) corresponding to the transmembrane domain of influenza HA (B, Rh-TMD) were incorporated into GUVs of different lipid compositions. (C and D) Full length HA tagged C-terminally with Cer (C, HA-Cer) or a HA fragment linked N-terminally to YFP and signal peptide (SP) (D, TMD-HA-YFP) were expressed in the plasma membrane of CHO-K1 cells. GPMVs were generated subsequently and used for confocal scanning microscopy. Rh, TAMRA. ∗Palmitoylation site.