Table 1.

DSC-derived mole fractions and average enthalpy change for the thermal denaturation of the higher order structure formed by the mutant c-MYC 23-mer sequence at a range of pH values

pH	Tm1°C	χ1	Tm2°C	χ2	Tm3°C	χ3	ΔH(kcal mol−1)
6.5	7.6 ± 0.5	0.93 ± 0.05	20.5 ± 0.2	0.07 ± 0.05	NA	NA	37 ± 1
6.0	21.7 ± 0.2	0.84 ± 0.05	32.5 ± 0.2	0.16 ± 0.05	NA	NA	43 ± 1
5.5	31.5 ± 0.2	0.38 ± 0.05	40.5 ± 0.2	0.54 ± 0.05	48.0 ± 0.2	0.08 ± 0.05	55 ± 2
5.0	38.1 ± 0.2	0.29 ± 0.05	46.6 ± 0.2	0.15 ± 0.05	49.5 ± 0.2	0.56 ± 0.05	54 ± 2
4.0	32.9 ± 0.2	0.26 ± 0.05	46.3 ± 0.2	0.74 ± 0.05	NA	NA	37 ± 1

Potassium concentration was 130 mM [K⁺] BPES, and pH values were 4.0, 5.0, 5.5, 6.0, and 6.5. DSC thermograms were fit with two overlapping two-state transitions at pH 4.0, 6.0, and 6.5, and with three overlapping two-state transitions at pH 5.0 and 5.5. NA, not applicable.