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. Author manuscript; available in PMC: 2010 Jul 16.
Published in final edited form as: J Biol Chem. 2007 Jun 22;282(34):24591–24598. doi: 10.1074/jbc.M611390200

FIGURE 2. Binding of COMP/TSP5 to heparin-Sepharose.

FIGURE 2

Serum-free COMP/TSP5-conditioned medium was loaded on to a heparin-Sepharose column. Unbound material was collected as flow-through. After binding, the column was washed with TBS/C containing 0.15 M NaCl. The bound material was eluted with TBS/C containing 0.55 M NaCl.