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. Author manuscript; available in PMC: 2010 Jul 16.
Published in final edited form as: J Biol Chem. 2007 Jun 22;282(34):24591–24598. doi: 10.1074/jbc.M611390200

FIGURE 5. Competition of GAGs for aggrecan binding to COMP/TSP5.

FIGURE 5

The solid-phase binding assay was carried out as described in the legend to Fig. 3 with COMP/TSP5 coated in the presence of calcium. GAGs at a final concentration of 0.5 mg/ml were incubated with coated COMP/TSP5 before aggrecan was added to the plate. The binding was expressed as % binding of COMP/TSP5 to aggrecan without any GAG competitors.