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. 2010 May 28;61(12):3245–3258. doi: 10.1093/jxb/erq146

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 4. — Total cell-wall pectin-content (A), its degree of methylation (B), and unmethylated pectin content (C) in 1 cm root tips of Al-sensitive common bean genotype (VAX 1). Plants were pre-treated without or with 150 g l⁻¹ PEG in a simplified solution (pH 4.5) containing 5 mM CaCl₂, 1 mM KCl, and 8 μM H₃BO₃ for 24 h, then 30 root tips (1 cm) were harvested and cell-wall material was isolated according to Method A described in the Materials and methods for the determination of pectin content and degree of methylation. Bars represent means ±SD, n=4. Means with the same letters are not significantly different at P <0.05 (t test).