Fig. 10.

Recapitulation of sue4 phenotype in the wild type by overexpressing At3g55880 cDNA. (A) Illustration of the overexpression construct pCB2004-SUE4 and verification of the overexpression transgenic line (ox) via RT-PCR analysis. Tubulin was used as loading control. (B) Root elongation assay. The wild type (wt), sue4, and the ox seeds were vertically germinated and grown for 12 d on 1/2× MS medium and sulphur-free medium (–S) before primary root length was measured. (C) Primary root length after 12 d growth as in (B). Values represent the mean of >30 plants and error bars represent SEM. *0.05 < P <0.01, **P <0.01, ***P <0.001. (This figure is available in colour at JXB online.)