Fig. 11.

Overexpression of SUE4 in tobacco recapitulates sue4-like phenotype. (A) Verification of overexpression transgenic lines in tobacco via RT-PCR analysis. Tubulin was used as loading control. (B) Root elongation assay. The pCB2004-SUE4 was used to generate transgenic tobacco plants. The overexpression line 4-6, 9-6, 9-7, and the wild type were assayed for primary root elongation on 1/2× MS medium (normal) and sulphur-free medium. Primary root length was measured at day 20 after sowing. (C) Primary root length after 20 d growth as in (A). Values represent the mean of >50 plants and error bars represent SEM. *0.05 < P <0.01, **P <0.01, ***P <0.001. (D) Survival ratio under oxidative stress. The wild type, 4–6, and 9–7 were co-planted on MS medium and MS containing 5 μM paraquat. Values represent the mean of >50 plants and error bars represent SEM. *0.05 <P <0.01, **P <0.01, ***P <0.001. (E) Survival ratio under Cd stress. The wild type, 4–6, and 9–7 were co-planted on MS medium and MS containing 100 μM CdCl₂ in hydroponic system. Values represent the mean =20 plants and error bars represent SEM. *0.05 <P < 0.01, **P <0.01, ***P <0.001. (This figure is available in colour at JXB online.)