Growth behaviour of sue3, sue4, and the wild type under normal conditions and hydroponic culture. (A) 8-d-old seedlings grown on MS medium, sue3 versus wt (upper) and sue4 versus wt (lower). (B) 2-week-old plants grown in soil, sue3 versus wt (upper) and sue4 versus wt (lower). (C) 4-week-old plants grown in soil, sue3 versus wt (upper) and sue4 versus wt (lower). (D) 7-week-old plants grown in soil, sue3 versus wt (upper) and sue4 versus wt (lower). (E) After 3 weeks grown in hydroponics system with sulphate concentration of 75 μM, the wild type and the sue3 began to bolt, while sue4 was delayed. (F) Bolting frequency under low sulphur stress. The wild type (wt) and sue4 seeds were sown on 1/2× MS medium (750 μM sulphate) and low sulphur medium (75 μM sulphate) for 7 d, and the contents in the low sulphur medium is the same as in the 1/2× MS medium except the sulphur concentration. Then seedlings were translated in hydroponics system containing 1/2× MS medium and low sulphur medium for 3 weeks. Values represent the mean of >20 seedlings. Error bars represent SEM. *0.05< P <0.01, **P <0.01, ***P <0.001. (This figure is available in colour at JXB online.)