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. 2010 Jun 21;61(12):3423–3437. doi: 10.1093/jxb/erq164

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 4. — BY-2 cells overexpressing OsKCH1 are more elongated than wild-type cells. (A) Expression of KCH in non-transformed BY-2 wild-type cells (Wt, white) and BY-2 cells stably overexpressing OsKCH1 (KCH1, black). (B) Representative RT-PCR detection of KCH expression levels in BY-2 Wt and BY-2 KCH1. Ubiquitin (Ubq) was used as internal standard in all samples. (C) Cell length in BY-2 Wt and BY-2 KCH1. Mean values ±SE of a total of 300 analysed cells per sample, collected cumulatively in three independent experimental series are given. Asterisks (**) indicate P <0.01 as evaluated by a t test for unpaired data. (D) Cell width in BY-2 Wt and BY-2 KCH1. For details refer to (C). (E) Representative DIC images of (a) BY-2 Wt and (b) BY-2 KCH1 cells to show the differences in cell length. Scale bars: 20 μm.