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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Cell Host Microbe. 2009 Dec 17;6(6):513–522. doi: 10.1016/j.chom.2009.11.004

Fig. 5. HCV infection strongly enhances phosphorylation of PKR and eIF2α proteins in IFNβ-treated cells.

Fig. 5

Huh-7 cells were infected with JFH-1 virus at moi=0.2. At day 5 post-infection uninfected (−) and infected (+) cells were treated for 16 hours with different doses of IFNβ. Total cellular protein in extracts prepared in RIPA buffer was quantified by BCA. Equivalent amounts from each sample were subjected to Western-blot analysis for the detection of viral core and cellular eIF2α, p-eIF2α, PKR and p-PKR proteins. β-actin expression was examined as protein loading control. The figure is representative of 2 independent experiments with 3 replicas per sample.