Figure 4.

Analysis of the production and RNA binding of Prp43p variants. (A) Coomassie blue staining (left) and western blot analysis (right) using anti-histidine antibodies of purified histidine-tagged wild-type Prp43p and the indicated histidine-tagged Prp43p variants. (B) Band retardation assays performed with the 5′ half of snR5 snoRNA at 1 nM hybridized to a 21 nucleotide-long radiolabelled ssDNA (Lebaron et al, 2009) and wild-type Prp43p (left), Prp43pΔL12 (middle) or Prp43p(1–657) (right) at the indicated concentrations.