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Prp43p(1–657) fails to interact with the G-patch-containing domain of Pfa1p. The indicated combinations of His-tagged wild-type or truncated Prp43p and Pfa1p proteins were incubated at 33 nM for the wild type and 130 nM for the truncated proteins and precipitated with anti-Prp43p antibodies. Proteins in the pellet (IP) and supernatant (Sup) fractions were detected by western blot analysis using anti-histidine antibodies.
