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. 2010 May 28;29(13):2173–2181. doi: 10.1038/emboj.2010.108

Figure 4.

Figure 4

Cid14p is most likely not involved in the Mmi1-dependent selective elimination of the meiotic mRNAs (A) JY450 (WT), JT432 (rrp6-ts32) and JT654 (cid14Δ) cells were cultured at 25°C in YE medium, and then shifted to 37°C. At the indicated times, total RNA was extracted from each sample and processed for northern blot analysis. (B) JY450 (WT), JZ464 (sme2Δ), JT654 (cid14Δ) and JT655 (cid14Δ sme2Δ) cells were cultured in YE medium at 30°C and spotted onto an SPA plate. Incubation was continued at 30°C for 1 day, and then cells were stained with iodine and the sporulation efficiency was calculated. (C) JT432 (rrp6-ts32) and JT656 (rrp6-ts32 cid14Δ) cells were cultured at 25°C in YE medium, and then shifted to 37°C. At the indicated times, total RNA was extracted from each sample and processed for northern blot analysis.