Figure 6.

Analysis of heteromeric Kv1.x channels containing Kv1.1^I400V subunits. (A, B) The effects of (A) Psora-4 and (B) AA measured after co-expression of Kv1.1 and Kv1.1^I400V channels at different ratios in Xenopus oocytes. The left-hand panels show typical currents measured at +40 mV. The right-hand panels show the percentage of inhibition by (A) Psora-4 and (B) AA, observed after co-expression of different ratios of WT and Kv1.1^I400V subunits. Grey lines and squares indicate the inhibition expected for a binomial distribution; ** indicates P<0.01. (C–E) Currents measured after expression of heteromeric channels in oocytes; equal amounts of cRNA of Kv1.1^I400V and Kv1.x were co-injected. (C) The panel shows typical currents measured at +40 mV for Kv1.5 channels alone or co-expressed with Kv1.1 or Kv1.1^I400V subunits. (D) The effects of Psora-4 on homomeric Kv1.x channels and on heteromeric Kv1.x channels containing either WT Kv1.1 or Kv1.1^I400V. The concentration of Psora-4 was 2 μM for Kv1.3 and Kv1.4 and 500 nM for all other Kv1.x channels; ** indicates P<0.01. (E) AA sensitivity of homomeric Kv1.x channels and heteromeric Kv1.x channels containing edited Kv1.1^I400V subunits, analysed at +40 mV; ** indicates P<0.01.