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. 2010 Jun 1;103(1):61–72. doi: 10.1038/sj.bjc.6605699

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Copyright © 2010 Cancer Research UK

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Total and phosphorylated protein levels. Trastuzumab treatment (from 24 to 96 h) inhibited MAPK and AKT EGF-induced-phosphorylation of OVCAR-3WT and A cell lines under serum-starvation conditions. (A) Western blot analysis showing in OVCAR-3LTE cells: inhibition of the active form of PTEN and total p27^kip, upregulation of phosphorylated MEK1/2, and higher RhoB protein level expression (B).