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. 2010 Jun 1;103(1):61–72. doi: 10.1038/sj.bjc.6605699

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Copyright © 2010 Cancer Research UK

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(A) Western blot analysis showing that inhibition of RhoB (shRNA-RhoB) in OVCAR-3LTE reverses the downregulation of phospho-PTEN and the upregulation of phospho-MEK1/2. Induction of RhoB (adeno-RhoB) in OVCAR-3A mimics the PTEN and MEK1/2 phosphorylation regulation observed in OVCAR-3LTE (panel A). (B) Histogram represents the quantisation of the statistically significant RhoB induction (adeno-RhoB, +88.9%) in OVCAR-3A and RhoB statistically significant downregulation (shRNA-RhoB, −53.11%) (panel B) observed by western blot (panel A). (C) Actin network. Inhibition of RhoB (shRNA-RhoB) in OVCAR-3LTE reverses actin cytoskeleton network organisation and density as observed in control cell lines (OVCAR-3A). Induction of RhoB (Adeno-RhoB) in OVCAR-3A cells mimics the actin cytoskeleton network organisation and density observed in OVCAR-3LTE cells (panel C). (D) Migration assay, when ‘scratch’ wounds were created by scraping to make an gap, RhoB inhibition (shRNA-RhoB) in OVCAR-3LTE restores migration capacities observed in control cell lines; on the contrary, induction of RhoB (Adeno-RhoB) in control cell lines (OVCAR-3A and OVCAR-3WT-data not shown) inhibits their migration as observed in long-time trastuzumab-exposed cell line OVCAR-3LTE (panel D).