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. Author manuscript; available in PMC: 2010 Jul 16.
Published in final edited form as: Biochemistry. 2002 Mar 26;41(12):3991–4001. doi: 10.1021/bi011922x

Figure 4.

Figure 4

Stimulated ESEEM spectra of HGGGW, PHGGGWGQ, and PrP(23–28, 57–91). (a) Spectra of unlabeled species with assignments, based on comparison to spectra obtained from the 15N-labeled peptides (see panel b), shown by the grids at the top. All spectra from unlabeled constructs show coupling to the remote NH of the imidazole and a noncoordinated amide nitrogen. (b) These spectra of 15N-labeled HGGGW and PrP(23–28, 57–91, 15N-64,72,80,88) show only those transitions due to the remote NH of the His imidazole consistent with the finding that the third glycine in each HGGGW is not coordinated to the Cu2+. Parameters for data acquisition were as follows: temperature, 4.2 K; π/2 pulse widths, 20 ns; repetition rate, 13 Hz; τ, 140 ns; T increment, 20 ns; 30 averages per point.