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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Cryobiology. 2009 Jun 18;59(2):223–225. doi: 10.1016/j.cryobiol.2009.06.008

Figure 2.

Figure 2

A comparison of hemoglobin glycation in whole blood from wood frog, R. sylvatica (●), leopard frog, R. pipiens (■) and rat (▲) during in vitro incubation at 23 °C with glucose. Blood was collected by aortic punch. Whole blood was mixed 1:1 with lysis buffer (Glyc-Affin GHb kit Sample Preparation Buffer), and glucose was added to a final concentration of 0.4 M. The samples were mixed well, tightly capped, and then incubated at 23 °C for up to 18 d on a rotary mixer. After the incubation period, GHb content was immediately analyzed with the Glyc-Affin GHb kit. Data are means ± SEM, n = 3 separate trials. (*)- Indicates a significant difference from the corresponding intraspecies control value at 0 d by ANOVA and post-hoc Student-Newman-Keuls test (two-tailed), P< 0.05. (#) and (§) - Indicates significant differences between R. sylvatica and the corresponding rat or R. pipiens, respectively, interspecies value at the same incubation time.