Figure 4.
Virulence of the ΔexbD and ΔyfeABCD mutants can be rescued by FeCl3. Overnight cultures were prepared and 1000 CFU of WT (diamonds), ΔexbD (squares) and ΔyfeABCD (triangles) were injected into 5th instar M. sexta larvae (open symbols) or larvae pre-injected with 10 μl of a solution containing 5 mM FeCl3 (filled symbols). The data shown is the mean of at least 2 independent experiments (with n = 10 insects/experiment). For clarity the standard deviations are not shown but these values were within expected limits (0-35%).