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. Author manuscript; available in PMC: 2010 Jul 16.
Published in final edited form as: Pharmacogenet Genomics. 2008 Dec;18(12):1059–1070. doi: 10.1097/FPC.0b013e3283131661

Fig. 3.

Fig. 3

Identification and qualification of the G15927A mutation in the mitochondrial tRNAThr gene. (a) Partial sequence chromatograms of tRNAThr gene from an affected individual (WZD32-III-2) and a married-in-control (WZD32-III-1). An arrow indicates the location of the base changes at position 15927. (b) The location of the G15927A mutation in the mitochondrial tRNAThr. Cloverleaf structure of human mitochondrial tRNAThr is derived from the study of Florentz et al. [39]. Arrow indicates the position of the G15927A mutation. (c) Quantification of G15927A mutation in the tRNAThr gene of mutants and controls derived from the Chinese families. PCR products around the G15927A mutation were digested with HpaII and analyzed by electrophoresis in a 7% polyacrylamide gel stained with ethidium bromide.