Figure 5.
Relative aminoacylation and editing activities of deletion mutants on the β-strands of E. coli LeuRS. A. N-terminal β-strand. B. C-terminal β-strand. The activity of aminoacylation (top) and amino acid editing (bottom) were normalized to wild type aminoacylation activity based on the kcat/KM (Table 1) and are shown in bar graph form. Estimates of the post-transfer editing activities were normalized by comparing the hydrolyzed Ile-tRNALeu at the two minute time point. The horizontal dashed line represents the activity of wild type E. coli LeuRS.