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. Author manuscript; available in PMC: 2010 Jul 16.
Published in final edited form as: J Biol Chem. 2005 Jun 8;280(32):29151–29157. doi: 10.1074/jbc.M504247200

Fig. 3. Expression analysis of mitochondrial genes.

Fig. 3

Equal amounts (20 μg) of total cellular RNA from various mutant and control strains were electrophoresed through a 1.8% agarose-formaldehyde gel and transferred onto a positively charged membrane. The blot was hybridized with a DIG-labeled CYTB RNA probe. After stripping the blot, it was hybridized with DIG-labeled COX1, 15 S RNA, and 21 S rRNA probes, respectively. Subsequently, after restripping of the blot, it was hybridized with a DIG-labeled nuclear 25 S RNA probe as an internal control.