Skip to main content
. Author manuscript; available in PMC: 2010 Jul 16.
Published in final edited form as: J Biol Chem. 2005 Jun 8;280(32):29151–29157. doi: 10.1074/jbc.M504247200

Fig. 6. Properties and expression of human gene homolog of yeast MTO2.

Fig. 6

A, subcellular localization of hMto2p in human 143B cells. Cells were transiently transfected with a MTO2 cDNA fused with GFP or pEGFP. The fusion protein was visualized by indirect immunofluorescence using antibodies to human COX1 and to GFP. A merged image is shown on the right. B, series dilutions of the wild type and mutant strains (the null mto2 strain carrying the C1409G allele transformed with the pDB20-HMTO2 and pDB20 vector) were spotted on glucose and glycerol media, respectively. The plates were then incubated at 30 °C in glucose medium for 3 days and in glycerol medium for 5 days.