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ROS production was measured by DCF fluorescence in microglial cells. Microglial cells were treated with MeHg at the concentrations indicated in the graph; 100μM H2O2 was used as a positive control. DCF fluorescence levels were measured after 1 and 10 min treatment, respectively. The results demonstrated that higher MeHg concentration and longer exposure time led to higher ROS level. Values are expressed as the mean ± SEM derived from six independent experiments.
