FIG. 5.

GSH and GSSG levels were measured by HPLC after MeHg treatment. Microglial cells were treated with MeHg at the concentrations indicated in the graph for 1 min, 10 min, and 1 h; 100μM H₂O₂ was used as a positive control. The ratios of GSH:GSSG were calculated, and the percentages of control were used to plot the graph. Each treatment group was compared with its corresponding control level. The results demonstrated that MeHg caused acute and robust reduction in microglial GSH level in a concentration- and time-dependent manner. Values are expressed as the mean ± SEM derived from three independent experiments.