FIG. 8.
The expression of Ho-1, Nqo1, and xCT was measured by real-time PCR after MeHg treatment. Microglial cells were treated with MeHg at the concentrations indicated in the graph for 30 min; 100μM H2O2 was used as a positive control. The mRNA levels of Ho-1 (A), Nqo1 (B), and xCT (C) were measured by real-time PCR. The differences in the average threshold cycle (ΔCt) values were determined and normalized to the expression of β-actin. Values are expressed as the mean ± SEM derived from five independent experiments.