FIG. 1.

Effects of SFN and PEITC on AFB-DNA adduct formation in human hepatocytes. Hepatocytes were treated with two concentrations of each phytochemical for 48 h and subsequently coincubated with 0.4μM ³H-AFB and phytochemical. Control represents nonmodulated AFB-DNA adduct level, where cells were treated with vehicle instead of phytochemical. AFB-DNA adduct levels are expressed as percentages of control and represent means and SEs from six independent experiments (i.e., hepatocytes from six donors). The 100% control value for six hepatocyte preparations was 5.9 adducts per 10⁷ nucleotides (range 2.2–10.7 adducts per 10⁷ nucleotides). “*” Denotes 0.01 < p < 0.05; “**” denotes p < 0.01 comparing vehicle control (100%) versus phytochemical incubations.