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. 2010 May 4;116(2):422–432. doi: 10.1093/toxsci/kfq135

FIG. 2.

FIG. 2.

Effects of SFN on CYP3A4 mRNA expression in cultured human primary hepatocytes over 96 h. CYP3A4 mRNA levels of hepatocytes were determined at the following times and treatments: (A) at 0 h (no DMSO or SFN treatment); (B) following 24 h treatment with 10 or 25μM SFN or DMSO; (C) following 48 h of SFN or DMSO treatment; (D) 48 h in culture with DMSO (no SFN) followed by 24 h of SFN or DMSO treatment; (E) 48 h in culture with DMSO (no SFN) followed by 48 h of SFN or DMSO treatment. Bars represent means of normalized CYP3A4 mRNA expression, including corresponding SD from three individual cell culture vessels from a single hepatocyte donor. “*” Denotes p < 0.05 comparing vehicle control versus SFN incubations within each condition.