TABLE 1.

Voltage-Dependent Activation Parameters in PC12 Cells

	V50 (mV)	k
Control	−5.5 ± 2.5	4.7 ± 0.5
5μM AL	−9.5 ± 3.4	3.8 ± 1.0
TTX	−9.9 ± 2.9	3.2 ± 1.4
TTX + AL	−8.6 ± 2.6	5.1 ± 1.3
GVIA	−6.2 ± 3.1	4.8 ± 0.7
GVIA + AL	−12.0 ± 3.5a	5.2 ± 0.4
NIM	−2.1 ± 3.5	3.6 ± 0.9
NIM + AL	−0.90 ± 3.4	4.4 ± 0.5

Note. Parameters were estimated using the Boltzman equation (G/G_max = 1/(1 + e^{−(V − V50)/k})), where V₅₀ is the half-activation voltage and k is a measure of steepness. No significant differences were observed within treatments, except that cells exposed to both GVIA and AL exhibited activation at a significantly more negative potential than cells exposed to GVIA alone. Values are reported as the mean ± SEM, n = 5–7 cells per condition.

^aSignificance from GVIA-treated cells (p < 0.05, paired t-test).