TABLE 2.

Voltage-Dependent Inactivation Parameters in PC12 Cells

	V50 (mV)	k
Control	−0.1 ± 5.4	−32.6 ± 9.2
5μM AL	0.8 ± 5.2	−36.4 ± 8.3
TTX	−9.5 ± 9.4	−29.8 ± 4.9
TTX + AL	−9.8 ± 5.5	−31.2 ± 6.3
GVIA	40.4 ± 10.3a	−40.6 ± 17.3
GVIA + AL	−1.0 ± 7.4b	−45.3 ± 9.6
NIM	−13.4 ± 2.4	−27.8 ± 2.6
NIM + AL	−11.6 ± 3.9	−20.2 ± 5.0

Note. Parameters were estimated using the Boltzman equation (I/I_max = 1/(1 + e^{−(V − V50)/k})), where V₅₀ is the half-inactivation voltage and k is a measure of steepness. No significant differences were observed within treatments, except that cells exposed to both GVIA and AL exhibited inactivation at a significantly more negative potential than cells exposed to GVIA alone.

^aExtrapolated V₅₀ of inactivation, as full inactivation is likely only theoretical under these conditions. Values are reported as the mean ± SEM, n = 5–7 cells per condition.

^bSignificance from GVIA-treated cells (p < 0.05, paired t-test).