FIG. 4.

Real-time qPCR results confirming the up- and downregulated transcripts in Jurkat cells. Relative amount of transcripts with the highest fold difference (DKC1 and ERGIC3) and the lowest fold difference (PTP4A2 and ZAP70) from our screening. DKC1 and PTP4A2 are upregulated transcripts, whereas ERGIC3 and ZAP70 are downregulated transcripts. 18S was used to normalize the message amount. The error bar represents ±SD of three independent experiments [n = 3, †p > 0.05 (not significant) and ** p ≤ 0.005]. This experiment has been repeated at least once.