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. 2010 May 23;116(2):549–561. doi: 10.1093/toxsci/kfq150

FIG. 7.

FIG. 7.

Effect of BaP on Nrf2 target genes. Real-time qPCR results showing the effect of BaP (A) or 3MP-ITC (B) on the gstp1, nqo1, and gclc messages in Jurkat cells. Cells were treated with either 2.5μM BaP (48 h), 25μM 3MP-ITC (24 h) or vehicle DMSO. The error bar represents ±SD of three independent experiments (n = 3). BaP experiment has been repeated at least once. (C) Western data showing the upregulation of GSTP1 protein by treatment of either BaP (1.4-fold) or 3MP-ITC (1.3-fold). The images above show the Western images of three independent experiments. The plot below is the quantified data showing the means with the error bars (mean ± SD, n = 3). Ten micrograms of WCE was loaded in each lane, and GAPDH was used as the loading control. (D) Western data showing NQO1 expression in Hepa1c1c7 but not in Jurkat cells. Cells were treated with 2.5μM BaP or DMSO vehicle for 48 h. Twenty micrograms of WCE was loaded in each lane, and GAPDH was used as the loading control. The GSTP1 and NQO1 Western experiments have been repeated at least once. *p ≤ 0.05, **p ≤ 0.005, †p > 0.05 (not significant).