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. 2010 May 23;116(2):549–561. doi: 10.1093/toxsci/kfq150

TABLE 3.

Primer sequences for real-time qPCR. All primer sequences are from Primer Bank except human Keap1 (Ohta et al., 2008), GCLC (Aguirre et al., 2007), GSTP1 (Loignon et al., 2009), and NQO1 (Loignon et al., 2009)

Gene name Real-time qPCR primers
PTP4A2 Forward: 5′ TTTCGTGAAGAGCCAGGTTG 3′
Reverse: 5′ CGTAATCGCATCTTAGGTCGG 3′
DKC1 Forward: 5′ CCAAAGTTGCTAAGTTGGACACG 3′
Reverse: 5′ TGCAAGAGGTGTATAGTGTGTTG 3′
ZAP70 Forward: 5′ TGCCCTTCTTCTACGGCAG 3′
Reverse: 5′ ACGAGCGACAGCACATAGC 3′
ERGIC3 Forward: 5′ GGGAGGACTATCCAGGCATTG 3′
Reverse: 5′ AGCTCATAGAGGACGAAGACTC 3′
Human Keap1 Forward: 5′ TACGATGTGGAAACAGAGACGTGGACTTTCGTA 3′
Reverse: 5′ TCAACAGGTACAGTTCTGCTGGTCAATCTGCTT-3′
Mouse Keap1 Forward: 5′ TACGACGTGGAGACAGAGACCTGGACTTTCGTA 3′
Reverse: 5′ TCAGCAGGTACAGTTTTGTTGATCAATTTGCTT 3′
GCLC Forward: 5′ ATGGAGGTGCAATTAACAGAC 3′
Reverse: 5′ ACTGCATTGCCACCTTTGCA 3′
GSTP1 Forward 5′ CCCTACACCGTGGTCTATTTCC 3′
Reverse 5′ GAGGCTTTGAGTGAGCCCT 3′
NQO1 Forward 5′ TGAAGGACCCTGCGAACTTTC 3′
Reverse 5′ GAACACTCGCTCAAACCAGC 3′