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. 2010 Jul 16;5(7):e11621. doi: 10.1371/journal.pone.0011621

Figure 2. Accumulation of misfolded proteins induces mild ER stress.

Figure 2

(A and C) Immunoblots of IRE1α and PERK in HEK293T cells transfected with the indicated plasmids for 24 h. NHK, the unfolded form of α1-antitrypsin; p97-QQ, dominant negative form of p97-WT. ER-dsRed and GFP, negative control plasmids. HSP90, a position and loading control. (B and D) Quantitation of percent of phosphorylated IRE1α in total IRE1α protein in Phos-tag gels shown in A, C. Values are mean ± SEM *, P<0.05 using unpaired two-tailed Student's t-test. Representative data from at least three independent experiments shown.