Figure 4. Specific binding of Rv2745c to the clpC1 promoter and disruption of DNA binding by targeted Rv2745c mutation.

Binding reactions were performed with 200pmol of the MTB clpC1 promoter fragment as a probe. Lysate  =  MTB protein lysate; C  =  unlabeled probe competitor; NC  =  unlabeled nonspecific DNA; DBL  =  L24SR25D mutant form of Rv2745S.